Supporting Information Lysosome-Controlled Efficient ROS Overproduction against Cancer Cells with High pH-Responsive Catalytic Nanosystem
نویسندگان
چکیده
Experimental section Materials Cetyltr imethyl ammonium bromide (C16TAB), tetraethyl orthosilicate (TEOS), Fe3O4 NPs (ca. 20 nm) and 3,3',5,5'-tetramethylbenzidine (TMB) were obtained from Aladdin. Titanium potassium oxalate (K2TiO(C2O4)2·2H2O), hydrogen peroxide (H2O2, 30%) and potassium ferricyanide were purchased from Sinopharm Chemical Reagent Co., Ltd. Potassium ferrate (K2FeO4), 3-aminopropyltriethoxys ilane (APTES), fluorescein isothiocyanate (FITC), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) were from Sigma. Phosphate buffered saline (PBS, pH 7.4), RPMI-1640 medium, trypsin and fetal bovine serum (FBS) were obtained from Gibco. All chemicals were used as received without further purification. Milli-Q water was used in all experiments.
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